Product Details

Bioline ACCUZYME™ DNA Polymerase

Bioline ACCUZYME™ DNA Polymerase

Features

• High-fidelity coupled with high yield
• Amplifies fragments up to 5 Kb
• Available as a convenient pre-mixed, pre-optimized solution (ACCUZYME™ Mix)

ACCUZYME Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Product Description
ACCUZYME™ is a thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, offering high fidelity. ACCUZYME provides high fidelity with an error-rate of 3.0 x 10-6, to give blunt-ended amplicons of up to 5Kb in length.

ACCUZYME is supplied with 10x Reaction Buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+ ) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided.

The specificity and performance of ACCUZYME can be further improved with the use of 2x PolyMate Additive, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

Applications
• Ultra-high fidelity for subsequent cloning
• Blunt-end cloning

Components
• Accuzyme DNA Polymerase
• 10x AccuBuffer
• 50mM MgCl2 Solution

Unit Definition
One unit will incorporate 10 nmoles of dNTPs in 30min at 72°C.

Concentration
2.5u/µl

Storage Conditions
ACCUZYME can be stored for 12 months at -20°C.

Shipping Conditions
On Dry Ice or Blue Ice

Note
ACCUZYME is a trademark of Bioline.

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