Product Details

Bioline DNA Loading Buffer, 5x

Bioline DNA Loading Buffer, 5x

Features

The Bioline range of Colored Crystal DNA Loading Buffers are ready-to-use solutions premixed with bromophenol blue (Blue), cresol red (Red), orange G and xylene cyanol FF (TriColor). The dyes in the Colored Crystal DNA Loading Buffers migrate at different rates depending on the dye (fig.1) and the concentration of the agarose gel (see Dye Migration Table below). This allow users to monitor DNA migration, and therefore increase the versatility of their DNA analysis, by choosing the buffer most suited to their application.

Coloured DNA Loading Buffer:

Delivery time: 2 Weeks
Delivery times may vary
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Colored loading for easy recognition
No need to add dye
Guarantee reproducible results


Applications
To monitor migration rate during agarose electrophoresis
Loading of samples onto DNA agarose gels

Dye Mobility Table

The following table gives the approximate migration rates of dyes in different agarose concentrations in TAE buffer. The values indicate the size of DNA fragments with which the dye will co-migrate at that particular gel concentration.

 

 

AGAROSE GEL CONCENTRATION

XYLENE CYANOL FF

BROMOPHENOL BLUE

CRESOL RED

ORANGE G

0.7%

8000

600

3000

100

1.0%

4000

400

1500

500

1.5%

2000

250

900

20

2.0%

900

120

300

>10

3.0%

400

50

>100

>10

Storage Conditions

Crystal Colored 5x DNA Loading Buffers can be stored at -20°C for 24 months from the date of purchase.

Shipping Conditions

Dry or blue ice.

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