Product Details

Bioline dATP, 100mM (BIO-BIO-39036)

Features

• Ultra-pure: >99% trisphosphate by HPLC
• Shelf-life, useful for bulk purchasing
• Free from PCR inhibitors
• DNase, RNase and Nickase free
• Bulk and OEM sizes available

Conc:  100mM as 1 x 250µl


Delivery time: 2 Weeks
Delivery times may vary
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Product Description
Manufactured by Bioline in a purpose-built facility, ready-to-use molecular grade individual dNTP solutions at pH 7.5 supplied as lithium salts in purified water. For use in a wide range of applications including DNA polymerization reactions, DNA labeling, and sequencing processes.

For more information regarding Bulk, Custom and OEM dNTP services please visit our dedicated microsite.

Applications
Standard and long range PCR assays
cDNA synthesis
Real-time PCR
Low-copy assays
Microarrays
DNA sequencing
Labeling

Storage Conditions
Ultra-pure dNTPs can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.

Shipping Conditions
Shipped on Dry or Blue ice.

Physical constants, spectral and HPLC analysis
 

Product dATP Lithium 100M Solution
Nomenclature 2'-deoxyadenosine-5'-triphosphate
Formula C10H12N5O12P3Li4
Molecular Weight 514.916g/mol
λmax pH 7.0 259nm
ε at λmax 15.4 E x mmol-1 x cm-1
A250/A260 0.78 ± 0.03
A250/A260 0.15 ± 0.02
Concentration 100mM ± 2%
Appearance Clear Colorless Solution
pH of Solution 7.5
dNTP (HPLC Area) >99%
dNDP (HPLC Area) <1%
DNases, RNases, Nicking Activity Negative
Storage at –20°C
Stability >24 months

 

HPLC Profile of >99% pure dNTPs

dATP

1. Meijer, P-J., et al. Meth. Mol. Biol. 525(3), 1-17 (2009).
2. Dellinger, M., et al. Develop. Biol. 319(2), 309-320 (2008).
3. Hampson, L., et al. FEBS Lett. 581(21), 3955-3960 (2007).
4. Lloyd, R.E., et al. Gene. 172, 2515-2527 (2006).
5. Tabone, T., et al. NAR 34(6), e45 (2006).

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