Product Details

Bioline dTTP, 100mM (BIO-BIO-39039)

Features

• Ultra-pure: >99% trisphosphate by HPLC
• Shelf-life, useful for bulk purchasing
• Free from PCR inhibitors
• DNase, RNase and Nickase free
• Bulk and OEM sizes available

Conc:  100mM as 1 x 250µl


Delivery time: 2 Weeks
Delivery times may vary
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Description
Manufactured by Bioline in a purpose-built facility, ready-to-use molecular grade individual dNTP solutions at pH 7.5 supplied as lithium salts in purified water. For use in a wide range of applications including DNA polymerization reactions, DNA labeling, and sequencing processes.

For more information regarding Bulk, Custom and OEM dNTP services please visit our dedicated microsite.

Applications
• Standard and long range PCR assays
• cDNA synthesis
• Real-time PCR
• Low-copy assays
• Microarrays
• DNA sequencing
• Labeling

Storage Conditions
Ultra-pure dNTPs can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.

Shipping Conditions
Shipped on Dry or Blue ice.

Physical constants, spectral and HPLC analysis
 

Product dTTP Lithium 100mM Solution
Nomenclature 2'-deoxythymidine-5'-triphosphate
Formula C10H13N2O14P3Li4
Molecular Weight 505.903g/mol
λmax pH 7.0 267nm
ε at λmax 9.6 E x mmol-1 x cm-1
A250/A260 0.65 ± 0.03
A250/A260 0.73 ± 0.02
Concentration 100mM ± 2%
Appearance Clear Colorless Solution
pH of Solution 7.5
dNTP (HPLC Area) >99%
dNDP (HPLC Area) <1%
DNases, RNases, Nicking Activity Negative
Storage at -20°C
Stability >24 months


HPLC Profile of >99% pure dNTPs

dTTP

 

1. Meijer, P-J., et al. Meth. Mol. Biol. 525(3), 1-17 (2009).
2. Dellinger, M., et al. Develop. Biol. 319(2), 309-320 (2008).
3. Hampson, L., et al. FEBS Lett. 581(21), 3955-3960 (2007).
4. Lloyd, R.E., et al. Gene. 172, 2515-2527 (2006).
5.Tabone, T., et al. NAR 34(6), e45 (2006).

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