Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG.
Blue/white color screening
Induction of lac operon for protein expression
Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG
IPTG powder can be stored for 12 months at –20°C.
On Dry Ice or Blue Ice.
Prabhakar, V., et al. FEBS Lett. 583(6), 983-991 (2009).
Chan, C-H., et al. Conserv. Gen. 9(4), 1067-1070 (2008).
Hamblin, K., et al. Mol. Microbiol. 68(6), 1395–1405 (2008).
Maruta, F., et al. J. Drug Targeting 15(4), 311-319 (2007).
Ross, P.J., et al. Infect. Immun. 72(3), 1568-1579 (2004).
Meng, G. & Fütterer, K. Nat. Struct. Biol. 10, 935-941 (2003).
Maruta, F., et al. Can. Gene Therapy 9(6), 543-552 (2002).
No related products found.