Product Details

Bioline BioScript (BIO-BIO-27036)

Bioline BioScript

Features

Unrivalled stability
Working temperature range 37°- 45°C
Highly sensitive for enhanced cDNA yield
Produces high quality cDNA ideal for real-time PCR
Ultra-pure and DNase/RNase-free
Reverse transcribes RNA templates up to 9Kb

Bioline BioScript Pack Size:

Delivery time: 2 Weeks
Delivery times may vary
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Applications

First strand cDNA synthesis for real-time PCR
cDNA library construction
mRNA 5’ end mapping by primer extension
Dideoxynucleotide sequencing
End-labelling of DNA

Description

BioScript™ is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability and is active over a wide range of temperatures with no loss of activity following 1 week at room temperature. Unlike the wild-type enzyme, BioScript possesses low RNase H activity, which results in enhanced yields (Fig. 1 & 2). In addition, BioScript is highly sensitive even when the amount of template is a limiting factor.

BioScript is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of cDNA for PCR analysis of gene expression. BioScript can be used with total RNA, mRNA or in-vitro transcribed RNA.

 

Components
BioScript
5x Reaction Buffer

Concentration
200u/µl

Storage Conditions
BioScript can be stored for 12 months at -20°C.

Shipping Conditions
On Dry Ice or Blue Ice.

Product Citations

Corripio-Miyar, Y., et al. Mol. Immunol. 46(10), 2098-2106 (2009).
Chen, Y., et al. Blood 114(1), 40-48 (2009).
Le, H. K., et al. Can. Immunol. Immunother. 58(10), 1565-1576 (2009).
Østergaard, A.E., et al. Develop. Comp. Immunol. 33(4), 525-532 (2009).
Wang, T. and Secombes, J.C. Mol. Immunol. 45(5),1449-1457 (2008).
Roberts,K., et al. Plant Physiol. 145 (1), 230-235 (2007).
Shemesh,Y., et al. FASEB J. 21, 2304-2311(2007).
Kalinski, T., et al. Can. 106(9), 2028-2038 (2006).
Sneddon, S.F., et al. Endocrinol. 146(12), 5304-5312 (2005).
Zou, J., et al. Eur. J. Biochem. 271, 913-1923 (2004).

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