Product Details

BioAssay Systems EnzyChrom™ NADP/NADPH Assay Kit (BAS-ECNP-100)

Features

Quantitative determination of NADP and NADPH by colorimetric (565nm) method.


Delivery time: 2 Weeks
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Procedure 30 min
Kit size 100 tests
Detection limit 0.1 µM
Shelf life 6 months
Shipping On ice
Storage -20°C

 

Publications:
1.Du, J et al (2010). Mechanisms of ascorbate-induced cytotoxicity in pancreatic cancer. Clin Cancer Res 16(2):509-20. Assay: NADP/NADPH in Human MIA PaCa-2 panceatic cancer cells (Pubmed).

2.Summermatter, S et al (2010). Peroxisome proliferator-activated receptor {gamma} coactivator 1{alpha} (PGC-1{alpha}) promotes skeletal muscle lipid refueling in vivo by activating de novo lipogenesis and the pentose phosphate pathway. J Biol Chem 285(43):32793-800 begin_of_the_skype_highlighting              32793-800      end_of_the_skype_highlighting. Assay: NADP/NADPH in human, mouse tissue (Pubmed).

3.Korn, A et al (2009). Ferredoxin:NADP+ oxidoreductase association with phycocyanin modulates its properties. J Biol Chem 284(46):31789-97. Assay: NADP/NADPH in Cyanobacteria Synechocystis sp. (Pubmed).

4.Brown, SM et al (2010). Isocitrate dehydrogenase is important for nitrosative stress resistance in Cryptococcus neoformans, but oxidative stress resistance is not dependent on glucose-6-phosphate dehydrogenase. Eukaryot Cell 9(6):971-80. Assay: NADP/NADPH in Yeast Cryptococcus neoformans (Pubmed).

5.Domain, F et al (2007). Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol 66(2):383-94. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

6.Lee, HC et al (2009). Thymidine production by overexpressing NAD+ kinase in an Escherichia coli recombinant strain. Biotechnol Lett 31(12):1929-36. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

7.Lee, HC et al (2010). High NADPH/NADP+ ratio improves thymidine production by a metabolically engineered Escherichia coli strain. J Biotechnol 149(1-2):24-32. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

8.Lee, WH et al (2011). Enhanced production of GDP-L: -fucose by overexpression of NADPH regenerator in recombinant Escherichia coli. Appl Microbiol Biotechnol 91(4):967-76. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

9.Siedler, S et al (2011). Increased NADPH availability in Escherichia coli: improvement of the product per glucose ratio in reductive whole-cell biotransformation. Appl Microbiol Biotechnol. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

10.Tseng, HC et al (2009). Metabolic engineering of Escherichia coli for enhanced production of (R)- and (S)-3-hydroxybutyrate. Appl Environ Microbiol 75(10):3137-45. Assay: NADP/NADPH in Bacteria E.coli (Pubmed).

11.Guo, S et al (2009). Specific inhibition of hypoxia inducible factor 1 exaggerates cell injury induced by in vitro ischemia through deteriorating cellular redox environment. J Neurochem 108(5):1309-21. Assay: NADP/NADPH in Human neuroblastoma cells (Pubmed).

12.Liu, M et al (2009). Cardiac Na+ current regulation by pyridine nucleotides. Circ Res 105(8):737-45. Assay: NADP/NADPH in Human HEK human embryonic kidney cells (Pubmed).

13.Lui, VW et al (2010). An RNA-directed nucleoside anti-metabolite, 1-(3-C-ethynyl-beta-d-ribo-pentofuranosyl)cytosine (ECyd), elicits antitumor effect via TP53-induced Glycolysis and Apoptosis Regulator (TIGAR) downregulation. Biochem Pharmacol 79(12):1772-80. Assay: NADP/NADPH in Human Nasopharyngeal cancer cell lines (Pubmed).

14.Osada-Oka, M et al (2010). Glucose is necessary for stabilization of hypoxia-inducible factor-1alpha under hypoxia: contribution of the pentose phosphate pathway to this stabilization. FEBS Lett 584(14):3073-9. Assay: NADP/NADPH in Human Hepatocellular carcinoma cell line Hep3B (Pubmed).

15.Tang, GJ et al (2011). Novel role of AMP-activated protein kinase signaling in cigarette smoke induction of IL-8 in human lung epithelial cells and lung inflammation in mice. Free Radic Biol Med 50(11):1492-502. Assay: NADP/NADPH in Human Bronchial epithelial cells (Pubmed).

16.Cao, X et al (2010). The effects of quercetin in cultured human RPE cells under oxidative stress and in Ccl2/Cx3cr1 double deficient mice. Exp Eye Res 91(1):15-25. Assay: NADP/NADPH in Mouse Serum (Pubmed).

17.Csak, T et al (2011). Deficiency in myeloid differentiation factor-2 and toll-like receptor 4 expression attenuates nonalcoholic steatohepatitis and fibrosis in mice. Am J Physiol Gastrointest Liver Physiol 300(3):G433-41. Assay: NADP/NADPH in Mouse liver tissue extract (Pubmed).

18.Ding, X et al (2009). Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50(10):4957-66. Assay: NADP/NADPH in Mouse Serum (Pubmed).

19.Matsunami, T et al (2011). Enhancement of reactive oxygen species and induction of apoptosis in streptozotocin-induced diabetic rats under hyperbaric oxygen exposure. Int J Clin Exp Pathol 4(3):255-66. Assay: NADP/NADPH in Rat Pancreas (Pubmed).

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