Product Details

Bioline ISOLATE II Plant miRNA Kit 25 Reactions (BIO-BIO-52084)

Features

ISOLATE II Plant miRNA Kit is specially designed for the rapid, phenol-free isolation of high quality and highly enriched small RNA (<200nt) without bias, from a wide range of plant cells and tissues. Isolate high quality large RNA >200nt in parallel from the same sample.

Ideal for highly sensitive applications.

  • Rapid, phenol-free protocol - unbiased small RNA isolation
  • Novel separation matrix - highest yields of pure miRNA and small RNA
  • Highly specific small RNA enrichment: capture all small RNA species (<200nt) from plant cells and tissues
  • Parallel high quality large RNA (>200nt) purification from same sample
  • BioFilter and DNase I included - enhanced sample homogenization and efficient elimination of genomic DNA
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Description

Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are important regulators of plant development and gene expression. The isolation of high-quality small RNAs is the first step in the study of its expression and function analysis.

ISOLATE II Plant miRNA Kit is based on the latest in rapid spin column technology using a novel RNA silica as the separation matrix for the isolation of high quality and highly enriched small RNA species (<200nt) including miRNA, siRNA and tRNA. Small RNA are preferentially purified from other cellular components without the use of phenol or chloroform. High quality large RNA (>200nt) can be sequentially purified from the same sample. The kit is compatible with a wide range of plant cells and tissues (Fig. 1).

ISOLATE II Plant miRNA Kit has been developed to overcome the bias observed with small RNA isolation using phenol-based techniques, due to its proprietary small RNA separation and enrichment technology, as well as highly optimized chemistry.

ISOLATE II Plant miRNA kit allows convenient processing of multiple samples in 25 minutes, utilizing a two-column system to enrich exclusively for small RNAs. RNAs greater than approximately 200 nucleotides are bound to the first column and ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the second column. Once bound to the second column, small RNA molecules are then eluted, ready-for-use in a wide variety of downstream applications.

Selected plant samples isolated with the ISOLATE II Plant miRNA Kit

  Plants Tissue type
   
Tobacco (Nicotiana tabacum) Leaf, stem, root
Cotton (Gossypium) Leaf, cotton
Soy bean Leaf, stem, root
Potato (Solanum tuberosum) Leaf, tuber
Corn/Maize (Zea mays) Leaf, corn
Rice (Oryza sativa) Leaf
Thale cress (Arabidopsis thaliana) Leaf, stem
Tomato (Lycopersicon esculentum) Leaf
Strawberry Leaf, fruit, flower
Blackberry Leaf, berry
Apple (Malus sp.) Leaf, flower, pollen
Plum (Prunus sp.) Leaf, fruit
Peach (Prunus persica) Leaf
Pear (Pyrus sp.) Leaf
Vanilla bean Bean
Herbs Leaf
Persimmon (Ebenaceae) Leaf
Grape vine (Vitis sp.) Leaf, grape, skin
Pepper (Capsicum annuum ) Leaf, seed
Citrus Leaf
Chrysanthemum Leaf
Palm (Arecaceae) (Elaeis guineensis) Leaf
Eastern white Red Cedar Leaf
Pine needle (Pinaceae) Needle
Cucumber Leaf
Rose gum (Eucalyptus grandis) Root
Salvia sclarea Root
Pseudocyphellaria crocata Leaf
Sorghum Leaf
Sugar cane Leaf
 

Components

Reagent

25 Reactions

ISOLATE II BioFilters

25

ISOLATE II Large RNA Removal Columns

25

ISOLATE II miRNA Columns

25

Collection Tubes (2ml)

50

Collection Tubes (2ml)

25

Elution Tubes (1.7ml)

50

Lysis Buffer RPX

30ml

Wash Buffer W1

38ml

DNase I Solution (RNase-free)

0.8ml

DNase I Reaction Buffer DRB

6ml

RNA Elution Buffer

6ml

Product Manual

1

Bench Protocol Sheet

1

 

Storage & Stability

The DNase I Solution should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.

 

Shipping conditions

Ambient temperature

 

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Isolation of RNA from:

  • Fresh plant cells and tissue
  • Frozen and lyophilized plant tissue
  • Parallel isolation of high quality large RNA >200nt from the same sample types. 


Fully compatible with miRNA real-time RT-PCR assays, microarrays, single-cell assays and next-generation RNA sequencing (small RNA-seq).

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