A robust, efficient proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.
Efficient – highly productive target amplification and removal of 3’ A overhangs from amplicons up to 5 kb in length
Accurate – possesses 3’ - 5’ proofreading exonuclease activity that delivers an error rate of 3.0 x 10-6 for increased PCR fidelity versus Taq DNA Polymerase
Sensitive – high-yield amplification from limiting amounts of human, animal and plant template DNA
Robust – developed for reliable amplification of even the most challenging targets, including genomic DNA and GC-rich amplicons
Convenient – advanced buffering system minimizes the requirements for PCR optimization thereby reducing time to results and eliminating the cost of unnecessary repeats
Simple – mix reduces pipetting burden for increased consistency in assay set-up and reduced contamination risk
ACCUZYME is recommended for all routine cloning applications. ACCUZYME is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6, and results in blunt-ended amplicons of up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.
ACCUZYME is supplied with a buffering system that provides ideal conditions for most assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated. Nevertheless, in circumstances where further optimization is required to improve PCR specificity and/or yield, ACCUZYME includes an additional vial of MgCl2. ACCUZYME Mix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured by the reduction in the number of pipetting steps that can lead to pipetting errors.