Product Details

4-Methylumbelliferyl-B-D-Glucuronide MUG Trihydrat (DUC-M1404.0100)

4-Methylumbelliferyl-B-D-Glucuronide MUG Trihydrat

Features

Cleavage of the substrate 4-MUG by a -glucuronidase activity leads to the generation of the flurorigenic product 4-MU, which can be visualized or detected by irradiation with UV light.

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4-Methylumbelliferyl--D-Glucuronide Trihydrate

MUG trihydrate
C16H16O9.3H2O=406.35

4-MUG, 4-Methylumbelliferyl--D-Glucuronide Trihydrate is a fluorescent substrate for -D-Glucuronidase (GUS) encoded by the gusA gene isolated originally from E. coli. Cleavage of the substrate 4-MUG by a -glucuronidase activity leads to the generation of the flurorigenic product 4-MU, which can be visualized or detected by irradiation with UV light.

The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts in conjunction with 4-MUG at a peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).

  • store dry at 2-8C
  • soluble in DMFO and DMSO
  • S: 22-24/25
  • CAS 6160-80-1

 

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