4-MUG, 4-Methylumbelliferyl-ß-D-Glucuronide Trihydrate is a fluorescent substrate for ß-D-Glucuronidase (GUS) encoded by the gusA gene isolated originally from E. coli. Cleavage of the substrate 4-MUG by a ß-glucuronidase activity leads to the generation of the flurorigenic product 4-MU, which can be visualized or detected by irradiation with UV light.
The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts in conjunction with 4-MUG at a peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).
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