Product Details

2-Nitrophenyl-B-D-Galactopyranoside 5 g (DUC-O1409.0005)

2-Nitrophenyl-B-D-Galactopyranoside 5 g

Features

ONPG is a colorimetric and spectrophotometric substrate for detection of -galactosidase activity.

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2-Nitrophenyl--D-Galactopyranoside

ONPG
C12H15NO8=301.26

ONPG is a colorimetric and spectrophotometric substrate for detection of -galactosidase activity.  ONPG is cleaved by -galactosidase via hydrolysis at the -1-4-glycosidic bond between 2-nitrophenol and galactose.  The released 2-nitrophenol is measured spectrophotometrically at 405 nm.  The absorbance intensity at this wavelength relates directly to the specific activity.

 

Assay >99%
 
  • store dry at -0C
  • soluble in DMSO and DMFO
  • protect from light and moisture
  • CAS 369-07-3

 

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