Product Details

5-Bromo-4-Chloro-3-Indolyl-b-D-Galactopyranoside, (DUC-X1402.0100)

5-Bromo-4-Chloro-3-Indolyl-b-D-Galactopyranoside,

Features

X-gal is a chromogentic substrate of -galactosidase. X-gal is used in conjunction with IPTG for detection of -galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue).

Delivery time: 2 Weeks
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5-Bromo-4-Chloro-3-Indolyl--D-Galactopyranoside

X-Gal
C14H15BrClNO6=408.6

X-gal is a chromogentic substrate of -galactosidase.  X-gal is used in conjunction with IPTG for detection of -galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue).   X-gal is cleaved at the 1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl part of X-gal by -galactosidase via hydrolysis.  The cleavage of X-gal results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage.  In cloning strategies with vectors like Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E.coli lacZ gene is transformed to lac cells.  After transformation of cells show -galactosidase activity in the presence of IPTG and X-gal containing media.  The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of -galactosidase activity leading to the appearance of white colonies on X-gal and IPTG containing media.  Non recombinant cells produce a blue indifo dye on these media.

 

Assay >90%
 
  • store dry at 2-8C or below
  • allow to warm to room temperature before opening
  • soluble in DMSO and DMFO
  • protect from light and moisture
  • S: 22-24/25
  • CAS 7240-90-6

 

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